Dr. Mélanie Auffan

Surface reactivity, Ecotoxicity, and Genotoxicity
of Engineered Nanomaterials during their life cycle 
Address: CEREGE, Europole de l'Arbois
13545 Aix-en-Provence, FRANCE

Phone: +33 (0)4 42 97 15 43
Fax: +33 (0)4 42 97 15 59


site duke

CEREGE: UMR 7330 CNRS/Aix-Marseille univ.
GDRi-iCEINT: international Consortium for the Environmental Implications of NanoTechnology
CEINT: Center for the Environmental Implications of NanoTechnology
Labex SERENADE: Safe(r) and Ecodesign Research and Education applied to Nanomaterial Development



Since 2009: CNRS research scientist, Aix en Provence, France 

Since 2013: Adjunct Assistant Professor, Civil and Environmental Engineering Department, Duke University, NC-USA

2007-2009: Research Associate, Civil and Environmental Engineering Department, Duke University, NC-USA

2004-2007: PhD in Geosciences of the Environment - Aix Marseille University 

2002-2004: Master's degree in Geosciences - Aix Marseille University  

2000-2002: Licence’s degree in Earth Science - Aix Marseille University



53. Tella M et al. Transfer, Transformation and Impacts of Ceria Nanomaterials in Aquatic Mesocosms Simulating a Pond Ecosystem. Environmental Science: Nano in press, 2015.

52. Greco F et al. Approach for the Oocyte Genotoxicity Assay: Adaptation of Comet Assay on Mouse Cumulus–Oocyte Complexes. Laboratory Animals 49: 251-254, 2015.

51. Bour A et al. Toxicity of CeO2 Nanoparticles on a Freshwater Experimental Trophic Chain: A Study in Environmentally Relevant Conditions through the Use of Mesocosms. Nanotoxicology online, 2015.

50. Preaubert et al. Cerium Dioxide Nanoparticles Affect in Vitro Fertilization in Mice. Nanotoxicology online, 2015.

49. Greco et al. Reprotoxicité Des Nanoparticules. Gynécologie Obstétrique & Fertilité 43: 49-55, 2015.

48. Bottero et al. Nanotechnology, Global Development in the Frame of Environmental Risk Forcasting. A Necessity of Interdisciplinary Researches. Comptes rendus geoscience 347: 35-42, 2015.

47. Barton L et al. Monte Carlo Simulations of the Transformation and Removal of Ag, Tio2, and Zno Nanoparticles in Wastewater Treatment and Land Application of Biosolids. Science of the Total Environment 511: 535-543, 2015.


46. Kumar et al. Molecular Insights of Oxidation Process of Iron Nanoparticles: Spectroscopic, Magnetic and Microscopic Evidences. Environmental Science & Technology 48: 9004-9013, 2014.


45. Collin et al. Environmental Release, Fate and Ecotoxicological Effects of Manufactured Ceria Nanomaterials. Environmental Science: Nano; 1: 533-548, 2014.

44. Benameur et al., DNA Damage and Oxidative Stress Induced by Ceo2 Nanoparticles in Human Dermal Fibroblasts: Evidence of a Clastogenic Effect as a Mechanism of Genotoxicity. Nanotoxicology; 1-10, 2014.

43. Fisichella et al., Toxicity evaluation of manufactured CeO2 nanoparticles before and after alteration: combined physicochemical and whole-genome expression analysis in Caco-2 Cells. BMC Genomics; 15: 700, 2014.

42. Secret et al., Two-photon excitation of porphyrin-functionalized porous silicon nanoparticles for photodynamic therapy. Advanced Materials; 26: 7643-7648, 2014.

41. Tella et al., Transfer, transformation and impacts of ceria nanomaterials in aquatic mesocosms simulating a pond ecosystem. Environmental Science & Technology; 48, 9004-9013, 2014

40. Santaella et al., TiO2-based nanocomposite used in sunscreens produces singlet oxygen under long-wave UV and sensitizes Escherichia coli to cadmium. Environmental Science & Technology 48, 5245-5253, 2014.

39. Barton et al., Theory and methodology for determining nanoparticle affinity for heteroaggregation in environmental matrices using batch measurements. Environmental Engineering Science; 31, 1-7, 2014.

38. Barton et al., Transformation of pristine and citrate-functionalized CeO2 nanoparticles in a laboratory-scale activated sludge reactor. Environmental Science & Technology; 48, 7289-7296, 2014.

37. Auffan et al., An adaptable mesocosm platform for performing integrated assessments of nanomaterial risk in complex environmental systems. Scientific reports; 4, 5608, 2014.

36. Auffan et al., Long-term aging of a CeO2 based nanocomposite used for wood protection. Environmental Pollution; 188, 1-7, 2014.

35. Auffan et al., Salinity-dependent silver nanoparticle uptake and transformation in Atlantic Killifish (Fundulus heteroclitus) embryos, Nanotoxicology; 8, 167-176, 2014


34. Courbiere et al., Ultrastructural Interactions and Genotoxicity Assay of Cerium Dioxide nanoparticles on Mouse Oocytes, International Journal of Molecular Sciences; 14, 21613-21628, 2013.

33. Artells et al., Exposure to Cerium Dioxide Nanoparticles Differently Affect Swimming Performance And Survival In Two Daphnid Species, PlosOne; 8, e71260, 2013

32. Leveques et al., Assessing ecotoxicity and uptake of metals and metalloids in relation to two different earthworm species (Eiseina hortensis and Lumbricus terrestris), Environmental pollution; 179, 232-241, 2013

31. Auffan et al., Role of molting on the biodistribution of CeO2 nanoparticles within Daphnia pulex, Water Research; 47, 3921-3930, 2013

30. Liu et al., Protein corona formation for nanomaterials and proteins of a similar size: hard or soft corona, Nanoscale; 5, 1658-1668, 2013


29. Liu et al., Influence of the length of Imogolite-Like nanotubes on their cytotoxicity and genotoxicity toward human dermal cells, Chemical Research in Toxicology; 25(11), 2513-2522, 2012

28. Auffan et al., Is there a Trojan horse effect during magnetic nanoparticles and metalloid co-contamination of human dermal fibroblasts?, Environmental Sciences & Technology; 46(19), 10789–10796 , 2012

27. Yang et al., Mechanism of silver nanoparticle toxicity is dependent on dissolved silver and surface coating in Caenorhabditis elegans, Environmental Sciences & Technology, 46(2), 1119–1127, 2012

26. Fisichella et al., Intestinal toxicity evaluation of TiO2 degraded surface-treated nanoparticles: a combined physico-chemical and toxicogenomics approach in caco-2 cells, Particles Fibers and Toxicology, 9(1); 18, 2012

25. Colman et al., Antimicrobial effects of commercial silver nanoparticles are attenuated in natural streamwater and sediment, Ecotoxicology, 21(7), 1867-1877, 2012

24. Kwok et al., Uptake of silvernanoparticles and toxicity to earlylifestages of Japanesemedaka (Oryzias latipes): Effect of coating materials, Aquatic Toxicology; 120-121; 59-66, 2012

23. Thill et al., Physico-chemical Control over the Single- or Double-Wall Structure of Aluminogermanate Imogolite-like Nanotubes, JACS;  134(8); 3780–3786, 2012

22. Thiery et al., Effects of metallic and metal oxide nanoparticles in aquatic and terrestrial food chains. Biomarkers responses in invertebrates and bacteria, International Journal of Nanotechnology; 9; 181-203, 2012

21. Masion et al., Environmental fate of nanoparticles: physical chemical and biological aspects – a few snapshot, International Journal of Nanotechnology; 9; 167-180, 2012


20. Hull et al., Filter-feeding bivalves store and biodeposit colloidally stable gold nanoparticles, Environmental Sciences & Technology; 45(15); 6592–6599, 2011

19. Gondikas et al., Early-stage precipitation kinetics of zinc sulfide nanoclusters forming in the presence of cysteine, Chemical Geology; 329, 10-17, 2011

18. Yin et al., More than the Ions: The Effects of Silver Nanoparticles on Lolium multiflorum, Environmental Sciences & Technology; 45(6); 2360–2367, 2011

17. Charlet et al., Reactivity at (nano)particle-water interfaces, redox processes, and arsenic transport in the environment, C. R. Geosciences; 343; 123–139, 2011

16. Bottero et al., Manufactured metal and metal-oxide nanoparticles: Properties and perturbing mechanisms of their biological activity in ecosystems, C. R. Geosciences; 343; 168–176, 2011

15. Botta et al., TiO2-based nanoparticles released in water from commercialized sunscreens in a life-cycle perspective: Structures and quantities, Environmental Pollution; 159(6); 1543-1550, 2011


14. Cotte et al., Environmental sciences at the ESRF, Synchrotron radiation news; 23(5), 2010

13. Meyer et al., Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans, Aquatic toxicology; 100(2); 140-50, 2010

12. Auffan et al., Inorganic manufactured nanoparticles: How their physico-chemical properties influence their biological effects in aqueous environments, Nanomedicine; 5(6); 999–1007, 2010

11. Auffan et al., Surface structural degradation of a TiO2-based nanomaterial used in cosmetics, Environmental Sciences & Technology; 44; 2689–2694, 2010

10. Labille et al., Aging of TiO2 nanocomposites used in sunscreen creams. Dispersion and fate of the byproducts in aqueous environment, Environmental Pollution; 158(12); 3482-3489, 2010


9. Zeyons et al., Direct and indirect CeO2 nanoparticles toxicity for E.coli and SynechocystisNanotoxicology; 3(4); 284-295, 2009

8. Auffan et al., Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective, Nature Nanotechnology; 3(4); 634-641, 2009

7. Kovochich et al., Comparative toxicity of C60 aggregates towards mammalian cells: role of the tetrahydrofuran (THF) decompositionEnvironmental Sciences & Technology; 43(16); 6378–6384, 2009

6. Auffan et al., CeO2 nanoparticles induce DNA damage towards human dermal fibroblasts in vitro, Nanotoxicology; 3(2); 161-171; 2009

5. Auffan et al., Chemical stability of metallic nanoparticles: a parameter controlling their potential cellular toxicity in vitro, Environmental Pollution; 157; 1127-1133; 2009


4. Auffan et al., Relation between the redox state of iron-based nanoparticles and their cytotoxicity towards Escherichia Coli, Environmental Sciences & Technology; 42(17); 6730–6735, 2008

2. Thill et al., Cytotoxicity of CeO2 Nanoparticles for Escherichia coli. Physico-Chemical Insight of the Cytotoxicity, Environmental Sciences & Technology; 40(14); 6151-6156, 2006


7.Auffan M et al., Impacts and Physico-Chemical Behavior of Inorganic Nanoparticles in the Environment. In Nanomaterials: A Danger or a Promise?, Brayner R, Fievet F, Coradin T, Springer, 2012

6. Auffan et al., Ecotoxicity of Inorganic Nanoparticles: From Unicellular Organisms to Invertebrates. In Encyclopedia of Nanotechnology, Bhushan B, Springer, 2012

5. Auffan et al., Surface reactivity of manufactured nanoparticles, in Nanosciences - Tome 4 - Nanotoxicology and NanoethicsSpringer, 2011

4. Auffan et al., Ecotoxicology: reactivity toward living organisms, in Nanosciences - Tome 4 - Nanotoxicology and Nanoethics, Springer, 2011

3. Auffan et al., Reactivite de surface des nanoparticules, in Nanosciences - Tome 4 - Nanotoxicologie et Nanoethique, Belin, 2010

2. Auffan et al., Ecotoxicologie: reactivite vis-a-vis des organismes vivants, in Nanosciences - Tome 4 - Nanotoxicologie et Nanoethique, Belin, 2010

1. Auffan et al., Nanoparticles as adsorbant. In Environmental Nanotechnology, Wiesner M, Bottero JY, McGraw-Hill, 2007


'C'Nano interdisciplinarity 2009', French C'nano network

'2009 Outstanding Postdoc', Duke university (USA)

'Young researcher 2008', Europole Mediterraneen de l'Arbois

'Hauy-Lacroix 2008', French Society of Mineralogy and Crystallography